Multiple sites of phosphorylation within the alpha heavy chain of Chlamydomonas outer arm dynein.
نویسندگان
چکیده
منابع مشابه
A Chlamydomonas outer arm dynein mutant missing the alpha heavy chain
A novel Chlamydomonas flagellar mutant (oda-11) missing the alpha heavy chain of outer arm dynein but retaining the beta and gamma heavy chains was isolated. Restriction fragment length polymorphism analysis with an alpha heavy chain locus genomic probe indicated that the oda-11 mutation was genetically linked with the structural gene of the alpha heavy chain. In cross-section electron microgra...
متن کاملStructure of the gamma heavy chain of the outer arm dynein from Chlamydomonas flagella
We describe here the vanadate-dependent photocleavage of the gamma heavy chain from the Chlamydomonas outer arm dynein and the pathways by which this molecule is degraded by endoproteases. UV irradiation in the presence of ATP, Mg2+, and vanadate cleaves the gamma chain at a single site (termed V1) to yield fragments of Mr 235,000 and 180,000. Irradiation in the presence of vanadate and Mn2+ re...
متن کاملMolecular analysis of the γ heavy chain of Chlamydomonas flagellar outer- arm dynein
We report here the complete sequence of the γ dynein heavy chain of the outer arm of the Chlamydomonas flagellum, and partial sequences for six other dynein heavy chains. The γ dynein heavy chain sequence contains four P-loop motifs, one of which is the likely hydrolytic site based on its position relative to a previously mapped epitope. Comparison with available cytoplasmic and flagellar dynei...
متن کاملA Chlamydomonas Outer Arm Dynein Mutant Missing the te Heavy Chain
A novel Chlamydomonas flagellar mutant (oda-11) missing the ot heavy chain of outer arm dynein but retaining the/3 and 3~ heavy chains was isolated. Restriction fragment length polymorphism analysis with an ct heavy chain locus genomic probe indicated that the oda-11 mutation was genetically linked with the structural gene of the tx heavy chain. In crosssection electron micrographs, the oda-ll ...
متن کاملIdentification of a Ca(2+)-binding light chain within Chlamydomonas outer arm dynein.
We describe here the molecular cloning of the M(r) 18,000 dynein light chain from the outer arm of Chlamydomonas flagella. In vivo, this molecule is directly associated with the gamma dynein heavy chain. Sequence analysis indicates that this light chain is a novel member of the calmodulin superfamily of Ca2+ binding regulatory proteins; this molecule is 42, 37 and 36% identical to calmodulin, c...
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ژورنال
عنوان ژورنال: Journal of Biological Chemistry
سال: 1994
ISSN: 0021-9258
DOI: 10.1016/s0021-9258(17)37707-4